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KMID : 0545119960060050336
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 5 p.336 ~ p.339
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Enzymatic Conversion of Glutaryl 7-Aminocephalosporanie Acid to 7-Aminocephalosporanic Acid with an Immobilized Glutaryl 7-Aminocephat Acid Acylase
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SHIN, HAN-JAE
LEE, SEUNG-GOO/LEE, WANG-SIK/YOON, KI-HONG
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Abstract
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Glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. SY-77-1 was immobilized with oxiran acrylic beads for the production of 7-aminocephalosporanic acid (7-ACA) from glutaryl 7-aminocephalosporanic acid (GL 7-ACA). The immobilized enzyme maintained its activity at a constant level for 7 days, but lost 30% of its activity after 20 days. Optimal reaction conditions for the synthesis of 7-ACA were found to be 30℃ and pH 8.0 using the immobilized enzyme. For the economic production of 7-ACA, substrate and enzyme concentrations were optimized to 60 mM and 0.5 g wet weight per 10 ml of reaction volume, respectively. Under optimized conditions, 50 mM 7-ACA was produced from 60 mM GL 7-ACA within 8 h, resulting in a conversion yield of 83%.
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